Osteopontin Polyclonal antibody
Osteopontin Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 25715-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue, HEK-293, C2C12 cells |
| Positive IHC detected in | human stomach cancer tissue, human kidney tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 25715-1-AP is Osteopontin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Osteopontin fusion protein Ag22588 |
| Full Name | secreted phosphoprotein 1 |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC007016 |
| Gene symbol | SPP1 |
| Gene ID (NCBI) | 6696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Osteopontin antibody 25715-1-AP | Download protocol |
| IHC protocol for Osteopontin antibody 25715-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment. | ||
ACS Biomater Sci Eng Magnesium Calcium Phosphate Cement Incorporating Citrate for Vascularized Bone Regeneration. | ||
Transplant Proc Resveratrol Ameliorated Vascular Calcification by Regulating Sirt-1 and Nrf2. | ||
Mol Med Rep Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts. | ||
J Inorg Biochem Citrate regulates extracellular matrix mineralization during osteoblast differentiation in vitro. | ||
Mol Med A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Eli (Verified Customer) (01-31-2020) | Loaded 20 (lanes 2 and 3) , 10 (lanes 4 and 5), 5 (lanes 6 and 7), and 2.5 (lanes 8 and 9) ug of cell lysates to the lanes listed.
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