Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25715-1-AP

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin



Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue, HEK-293, C2C12 cells
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25715-1-AP is Osteopontin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag22588
Full Name secreted phosphoprotein 1
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC007016
Gene symbol SPP1
Gene ID (NCBI) 6696
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

Protocols

Product Specific Protocols
WB protocol for Osteopontin antibody 25715-1-APDownload protocol
IHC protocol for Osteopontin antibody 25715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

EBioMedicine

Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment.

Authors - Rut Tejero
ratWB, IHC

ACS Biomater Sci Eng

Magnesium Calcium Phosphate Cement Incorporating Citrate for Vascularized Bone Regeneration.

Authors - Xiaopei Wu
humanWB

Transplant Proc

Resveratrol Ameliorated Vascular Calcification by Regulating Sirt-1 and Nrf2.

Authors - P Zhang
ratWB

Mol Med Rep

Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts.

Authors - Dan Liang
mouseWB

J Inorg Biochem

Citrate regulates extracellular matrix mineralization during osteoblast differentiation in vitro.

Authors - Xiaopei Wu
humanWB

Mol Med

A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone.

Authors - Yu Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eli (Verified Customer) (01-31-2020)

Loaded 20 (lanes 2 and 3) , 10 (lanes 4 and 5), 5 (lanes 6 and 7), and 2.5 (lanes 8 and 9) ug of cell lysates to the lanes listed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Osteogenically Differentiated hMSCs
Osteopontin Antibody Western Blot, validation (1:1000 dilution) in Osteogenically Differentiated hMSCs (Cat no:25715-1-AP)