Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 25715-1-AP


BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293, rat kidney tissue, HEK-293 cells, C2C12 cells
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25715-1-AP targets Osteopontin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag22588
Full Name secreted phosphoprotein 1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC007016
Gene Symbol SPP1
Gene ID (NCBI) 6696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).


Product Specific Protocols
WB protocol for Osteopontin antibody 25715-1-APDownload protocol
IHC protocol for Osteopontin antibody 25715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Eli (Verified Customer) (01-31-2020)

Loaded 20 (lanes 2 and 3) , 10 (lanes 4 and 5), 5 (lanes 6 and 7), and 2.5 (lanes 8 and 9) ug of cell lysates to the lanes listed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Osteogenically Differentiated hMSCs
Osteopontin Antibody Western Blot, validation (1:1000 dilution) in Osteogenically Differentiated hMSCs (Cat no:25715-1-AP)