|Positive WB detected in||mouse brain tissue, mouse cerebellum tissue|
|Positive IHC detected in||human cerebellum tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
28207-1-AP targets P2RX7 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P2RX7 fusion protein Ag27700|
|Full Name||purinergic receptor P2X, ligand-gated ion channel, 7|
|Calculated molecular weight||69 kDa|
|Observed molecular weight||70-80 kDa|
|GenBank accession number||BC011913|
|Gene ID (NCBI)||5027|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).
Adv Med Sci
The role of P2X7 receptor in prognosis and metastasis of colorectal cancer.
Biochem Biophys Res Commun
A438079 affects colorectal cancer cell proliferation, migration, apoptosis, and pyroptosis by inhibiting the P2X7 receptor.