P2RX7 Polyclonal antibody

• Featured Product
• KD/KO Validated

P2RX7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 28207-1-AP

Synonyms

Homo sapiens (Human), P2RX7, P2X7

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Validation Data Gallery

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  WB analysis using 28207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28207-1-AP (P2RX7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Product Information

28207-1-AP targets P2RX7 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P2RX7 fusion protein Ag27700
• Full Name: purinergic receptor P2X, ligand-gated ion channel, 7
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC011913
• Gene Symbol: P2RX7
• Gene ID (NCBI): 5027
• RRID: AB_2881088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publications

Species	Application	Title
mouse,rat	WB	J Cell Physiol; Apelin-13/APJ induces cardiomyocyte hypertrophy by activating the Pannexin-1/P2X7 axis and FAM134B-dependent reticulophagy.; Authors - Yiyuan Yang ; KD Validated
mouse,rat	WB,IHC,IF	Front Pharmacol; Salidroside Ameliorates Depression by Suppressing NLRP3-Mediated Pyroptosis via P2X7/NF-κB/NLRP3 Signaling Pathway.; Authors - Yuhui Chai
human	WB	Biochem Biophys Res Commun; A438079 affects colorectal cancer cell proliferation, migration, apoptosis, and pyroptosis by inhibiting the P2X7 receptor.; Authors - Ying Zhang
human	WB,IHC	Adv Med Sci; The role of P2X7 receptor in prognosis and metastasis of colorectal cancer.; Authors - Ying Zhang
human	WB	Biomed Res Int; Progesterone Reduces ATP-Induced Pyroptosis of SH-SY5Y Cells; Authors - Chang Cui
human,mouse	WB,IHC	Phytother Res; Asiaticoside hampers epithelial-mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer; Authors - Xuemei Huang