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P62,SQSTM1 Monoclonal antibody

P62,SQSTM1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


human and More (4)







Cat no : 66184-1-Ig


A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, Starvation treated HepG2 cells, Chloroquine treated U2OS cells, mouse brain tissue, rat brain tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66184-1-Ig targets P62,SQSTM1 in WB, IHC, IF, FC, CoIP applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, human, monkey, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.


Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
IHC protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
IF protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
FC protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


David (Verified Customer) (01-13-2020)

Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y