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P62/SQSTM1 Monoclonal antibody
P62/SQSTM1 Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Cat no : 66184-1-Ig
|Positive WB detected in||L02 cells, HEK-293 cells, HepG2 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Starvation treated HepG2 cells, mouse brain tissue, rat brain tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66184-1-Ig targets P62/SQSTM1 in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||bovine, human, monkey, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||P62/SQSTM1 fusion protein Ag13131|
|Full Name||sequestosome 1|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||62 kDa|
|GenBank accession number||BC017222|
|Gene ID (NCBI)||8878|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
|Product Specific Protocols|
|WB protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|IHC protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|IF protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|FC protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (01-13-2020)
Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.