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P62/SQSTM1 Monoclonal antibody

P62/SQSTM1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (3)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C1

Cat no : 66184-1-Ig

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3



Tested Applications

Positive WB detected inL02 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inStarvation treated HepG2 cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66184-1-Ig targets P62/SQSTM1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, human, monkey, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P62/SQSTM1 fusion protein Ag13131
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.

Protocols

Product Specific Protocols
WB protocol for P62/SQSTM1 antibody 66184-1-IgDownload protocol
IHC protocol for P62/SQSTM1 antibody 66184-1-IgDownload protocol
IF protocol for P62/SQSTM1 antibody 66184-1-IgDownload protocol
FC protocol for P62/SQSTM1 antibody 66184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

J Cell Physiol

Adiponectin inhibits high glucose-induced angiogenesis via inhibiting autophagy in RF/6A cells.

Authors - Rong Li
bovineWB,CoIP

Int J Mol Sci

Niacin Alleviates Dairy Cow Mastitis by Regulating the GPR109A/AMPK/NRF2 Signaling Pathway.

Authors - Wenjin Guo
WB

Acta Biochim Biophys Sin (Shanghai)

Parkin-induced ubiquitination of Mff promotes its association with p62/SQSTM1 during mitochondrial depolarization.

Authors - Ju Gao
humanIF

Neuropathology

Clinicopathological description of two cases with SQSTM1 gene mutation associated with frontotemporal dementia.

Authors - Gabor G Kovacs
mouseWB,IHC

Parasitol Res

In vivo and in vitro studies using Clonorchis sinensis adult-derived total protein (CsTP) on cellular function and inflammatory effect in mouse and cell model.

Authors - Mei Shang
monkeyWB

Biochem Biophys Res Commun

Rab11a is required for porcine reproductive and respiratory syndrome virus induced autophagy to promote viral replication.

Authors - Kai Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (01-13-2020)

Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y