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P62/SQSTM1 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66184-1-Ig |CloneNo.: 1H5C1

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Con: 200 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

P62/SQSTM1 Antibody WB HEK-293 cells 66184-1-Ig

WB result of P62/SQSTM1 antibody (66184-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells

P62/SQSTM1 Antibody WB L02 cells 66184-1-Ig

L02 cells were subjected to SDS PAGE followed by western blot with 66184-1-Ig( P62/SQSTM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours

P62/SQSTM1 Antibody IHC human liver cancer tissue 66184-1-Ig

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66184-1-Ig( P62/SQSTM1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P62/SQSTM1 Antibody IHC human liver cancer tissue 66184-1-Ig

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66184-1-Ig( P62/SQSTM1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P62/SQSTM1 Antibody IF Starvation treated HepG2 cells 66184-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed Starvation treated HepG2 cells using 66184-1-Ig(P62/SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

P62/SQSTM1 Antibody FC Jurkat cells 66184-1-Ig

1X10^6 Jurkat cells were stained with 0.20ug P62/SQSTM1 antibody (66184-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH

Species specificity:
human, monkey
Positive WB detected in:
L02 cells, HEK-293 cells, HepG2 cells
Positive IHC detected in:
human liver cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Starvation treated HepG2 cells
Positive FC detected in:
Jurkat cells
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P62/SQSTM1 fusion protein Ag13131
Full name:
sequestosome 1
Calculated molecular weight:
48 kDa
Observed molecular weight:
62 kDa
GenBank accession number:
BC017222
Gene ID (NCBI):
8878
Gene symbol
SQSTM1
Synonyms
A170, EBIAP, ORCA, OSIL, p60, P62, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

Background

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.


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