PAI-1 Monoclonal antibody

PAI-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

CloneNo.

1H4A5

Reviews (1)

Cat no : 66261-1-Ig

Synonyms

PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 66261-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66261-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66261-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig serum using 66261-1-Ig

  pig serum were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66261-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66261-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66261-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 66261-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 66261-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66261-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse lung using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66261-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (66261-1-Ig, Clone: 1H4A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, pig liver tissue, rat liver tissue, HuH-7 cells, U-251 cells, SH-SY5Y cells, pig serum, mouse liver tissue
• Positive IHC detected in: human kidney tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66261-1-Ig targets PAI-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAI-1 fusion protein Ag22411
• Full Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Calculated molecular weight: 402 aa, 45 kDa
• Observed molecular weight: 45-50 kDa
• GenBank accession number: BC010860
• Gene symbol: SERPINE1
• Gene ID (NCBI): 5054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Identification of Impacted Pathways and Transcriptomic Markers as Potential Mediators of Pulmonary Fibrosis in Transgenic Mice Expressing Human IGFBP5.; Authors - Xinh-Xinh Nguyen
human	WB	Am J Respir Cell Mol Biol; ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1.; Authors - Mingming Han
mouse	WB	Clin Sci (Lond); Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.; Authors - Mengqiu Wu
human	WB	Phytomedicine; Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3.; Authors - Tong Lin
human	IHC	Cancer Biomark; Weighted correlation network analysis identifies FN1, COL1A1 and SERPINE1 associated with the progression and prognosis of gastric cancer; Authors - Qiaoyun Zhao
mouse	WB,IHC	Arch Biochem Biophys; Human umbilical cord mesenchymal stem cell-derived exosomal microRNA-148a-3p inhibits neointimal hyperplasia by targeting Serpine1.; Authors - Xiaoyu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mona (Verified Customer) (10-17-2021)

Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: hamster (lung)