PAI-1 Monoclonal antibody

PAI-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, pig, mouse, rat







Cat no : 66261-1-Ig


PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1

Tested Applications

Positive WB detected inhuman peripheral blood platelets, pig liver tissue, rat liver tissue, HuH-7 cells, U-251 cells, SH-SY5Y cells, pig serum, mouse liver tissue
Positive IHC detected inhuman kidney tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66261-1-Ig targets PAI-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag22411
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC010860
Gene symbol SERPINE1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.


Product Specific Protocols
WB protocol for PAI-1 antibody 66261-1-IgDownload protocol
IHC protocol for PAI-1 antibody 66261-1-IgDownload protocol
IF protocol for PAI-1 antibody 66261-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

Identification of Impacted Pathways and Transcriptomic Markers as Potential Mediators of Pulmonary Fibrosis in Transgenic Mice Expressing Human IGFBP5.

Authors - Xinh-Xinh Nguyen

Am J Respir Cell Mol Biol

ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1.

Authors - Mingming Han

Clin Sci (Lond)

Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.

Authors - Mengqiu Wu


Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3.

Authors - Tong Lin

Cancer Biomark

Weighted correlation network analysis identifies FN1, COL1A1 and SERPINE1 associated with the progression and prognosis of gastric cancer

Authors - Qiaoyun Zhao

Arch Biochem Biophys

Human umbilical cord mesenchymal stem cell-derived exosomal microRNA-148a-3p inhibits neointimal hyperplasia by targeting Serpine1.

Authors - Xiaoyu Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Mona (Verified Customer) (10-17-2021)

Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: hamster (lung)