CoraLite®594-conjugated PAI-1 Monoclonal antibody

PAI-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H4A5

Cat no : CL594-66261

Synonyms

PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66261-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66261 targets PAI-1 in IF applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag22411
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC010860
Gene symbol SERPINE1
Gene ID (NCBI) 5054
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

Protocols

Product Specific Protocols
IF protocol for CL594 PAI-1 antibody CL594-66261Download protocol
Standard Protocols
Click here to view our Standard Protocols