CoraLite®594-conjugated PAI-1 Monoclonal antibody

PAI-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H4A5

Cat no : CL594-66261

Synonyms

PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66261

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated PAI-1 antibody (CL594-66261, Clone: 1H4A5 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66261 targets PAI-1 in IF applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAI-1 fusion protein Ag22411
• Full Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Calculated Molecular Weight: 402 aa, 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC010860
• Gene Symbol: SERPINE1
• Gene ID (NCBI): 5054
• RRID: AB_2883545
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.