PAICS Recombinant antibody, PBS Only (Capture)

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  WB analysis using 86255-2-RR (same clone as 86255-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86255-2-RR (PAICS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  Sample test of MP02343-1

  The mean PAICS concentration was determined to be 91.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02343-1

  Cytometric bead array standard curve of MP02343-1, PAICS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86255-2-PBS. Detection antibody: 86255-1-PBS. Standard: Ag3621. Range: 0.469-60 ng/mL.

PAICS Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86255-2-PBS

Clone No.250910H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

ADE2, ADE2H1, AIR carboxylase, AIRC, Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase

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Product Information

86255-2-PBS targets PAICS as part of a matched antibody pair:

MP02343-1: 86255-2-PBS capture and 86255-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAICS fusion protein Ag3621
• Full Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
• Calculated Molecular Weight: 425 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC010273
• Gene Symbol: PAICS
• Gene ID (NCBI): 10606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22234
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).