PAICS Recombinant antibody

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  WB analysis using 86255-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86255-2-RR (PAICS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PAICS Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86255-2-RR

Clone No.250910H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ADE2, ADE2H1, AIR carboxylase, AIRC, Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, HeLa cells, THP-1 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86255-2-RR targets PAICS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAICS fusion protein Ag3621
• Full Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
• Calculated Molecular Weight: 425 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC010273
• Gene Symbol: PAICS
• Gene ID (NCBI): 10606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22234
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).