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PAK1 Polyclonal antibody

PAK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 21401-1-AP


Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

Tested Applications

Positive WB detected inC6 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21401-1-AP targets PAK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAK1 fusion protein Ag16102
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Calculated Molecular Weight 553 aa, 62 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC109299
Gene Symbol PAK1
Gene ID (NCBI) 5058
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.


Product Specific Protocols
WB protocol for PAK1 antibody 21401-1-APDownload protocol
IHC protocol for PAK1 antibody 21401-1-APDownload protocol
IF protocol for PAK1 antibody 21401-1-APDownload protocol
IP protocol for PAK1 antibody 21401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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