PAK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PAK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 21401-1-AP

Synonyms

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

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Validation Data Gallery

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  WB analysis using 21401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21401-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21401-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 21401-1-AP

  IP result of anti-PAK1 (IP:21401-1-AP, 4ug; Detection:21401-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IHC staining of mouse brain using 21401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21401-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21401-1-AP (PAK1 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 21401-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21401-1-AP (PAK1 Antibody) at dilution of 1:50.

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  IF Staining of C6 using 21401-1-AP

  Immunofluorescent analysis of C6 cells using 21401-1-AP (PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C6 using 21401-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PAK1 antibody (21401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

21401-1-AP targets PAK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAK1 fusion protein Ag16102
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 1
• Calculated Molecular Weight: 553 aa, 62 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC109299
• Gene Symbol: PAK1
• Gene ID (NCBI): 5058
• RRID: AB_11232232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
mouse	WB	ACS Appl Mater Interfaces; Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.; Authors - Yi Li
mouse	IF,WB	Theranostics; Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.; Authors - Min Zhang
rat	WB	Acta Pharmacol Sin; Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway.; Authors - Cheng-di Liu
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang