PAN2 Polyclonal antibody

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  WB analysis of NIH/3T3 using 16427-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16427-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 16427-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PAN2 antibody (16427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

PAN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16427-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:3.1.13.4, hPAN2, Inactive ubiquitin carboxyl-terminal hydrolase 52, KIAA0710, PAB1P-dependent poly(A)-specific ribonuclease

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HepG2 cells
• Positive IHC detected in: mouse heart tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

16427-1-AP targets PAN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9670

  Product name: Recombinant human PAN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 850-1198 aa of BC024043

  Sequence: ATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLDAEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAAVFSSVLAL

• Full Name: PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
• Calculated Molecular Weight: 1198 aa, 135 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC024043
• Gene Symbol: PAN2
• Gene ID (NCBI): 9924
• RRID: AB_1851434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q504Q3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Nat Commun; USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.; Authors - Shangda Yang ; KD Validated
	WB	Nat Struct Mol Biol; An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.; Authors - Stefanie Jonas
human	IF	Neurobiol Dis; Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.; Authors - Rebecca L Kow
mouse	WB	Biol Reprod; Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.; Authors - Jun Ma
human	WB,IP	PLoS One; Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA.; Authors - Yi Hu
mouse	WB	Cells; Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).; Authors - Tian-Li Duan