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  • KD/KO Validated

PANX1 Polyclonal antibody

PANX1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 12595-1-AP


MRS1, pannexin 1, PANX1, PX1, UNQ2529

Tested Applications

Positive WB detected inJurkat cells, SH-SY5Y cells, K-562 cells, MDA-MB-231 cells, C2C12 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12595-1-AP targets PANX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PANX1 fusion protein Ag3174
Full Name pannexin 1
Calculated molecular weight 426 aa, 48 kDa
Observed molecular weight 45-50 kDa, 90 kDa
GenBank accession numberBC016931
Gene symbol PANX1
Gene ID (NCBI) 24145
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PANX1, also named as MRS1, belongs to the pannexin family. It plays a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis. It is a structural component of the gap junctions and the hemichannels. PANX1 induced the formation of intercellular channels with characteristics of gap junctions. PANX1 is 48-kDa protein which contains 4 transmembrane domains and cytoplasmic N and C termini. It is a mediator of find-me signal/nucleotide release from apoptotic cells. Western blot for Panx1 was also seen in two bands at 45~50 kDa and ~90 kDa. The band of ~90 kDa corresponding to the Panx1 dimer size. (PMID: 24590064, PMID: 19009624)


Product Specific Protocols
WB protocol for PANX1 antibody 12595-1-APDownload protocol
IHC protocol for PANX1 antibody 12595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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