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PANX1 Polyclonal antibody

PANX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12595-1-AP

Synonyms

MRS1, pannexin 1, PANX1, PX1, UNQ2529



Tested Applications

Positive WB detected inJurkat cells, SH-SY5Y cells, K-562 cells, MDA-MB-231 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12595-1-AP targets PANX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PANX1 fusion protein Ag3174
Full Name pannexin 1
Calculated molecular weight 426 aa, 48 kDa
Observed molecular weight 45-50 kDa, 90 kDa
GenBank accession numberBC016931
Gene symbol PANX1
Gene ID (NCBI) 24145
RRIDAB_2159186
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PANX1, also named as MRS1, belongs to the pannexin family. It plays a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis. It is a structural component of the gap junctions and the hemichannels. PANX1 induced the formation of intercellular channels with characteristics of gap junctions. PANX1 is 48-kDa protein which contains 4 transmembrane domains and cytoplasmic N and C termini. It is a mediator of find-me signal/nucleotide release from apoptotic cells. Western blot for Panx1 was also seen in two bands at 45~50 kDa and ~90 kDa. The band of ~90 kDa corresponding to the Panx1 dimer size. (PMID: 24590064, PMID: 19009624)

Protocols

Product Specific Protocols
WB protocol for PANX1 antibody 12595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Theranostics

Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.

Authors - Qing Wang
humanWB

Sci Signal

Structures of human pannexin-1 in nanodiscs reveal gating mediated by dynamic movement of the N terminus and phospholipids.

Authors - Maki Kuzuya
humanWB

PLoS Pathog

Defining the proteolytic landscape during enterovirus infection.

Authors - Mohsan Saeed
humanIHC

Front Oncol

Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma.

Authors - Xiaojie Song
mouseWB

Biochim Biophys Acta Mol Cell Res

TGF-β2 increases cell-cell communication in chondrocytes via p-Smad3 signalling.

Authors - Mengmeng Duan
humanWB, IHC

Sci Rep

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Authors - Chang Chen