PANX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PANX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 12595-1-AP

Synonyms

MRS1, pannexin 1, PANX1, PX1, UNQ2529

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Validation Data Gallery

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  WB analysis using 12595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12595-1-AP (PANX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12595-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12595-1-AP (PANX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, SH-SY5Y cells, K-562 cells, MDA-MB-231 cells, C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

12595-1-AP targets PANX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PANX1 fusion protein Ag3174
• Full Name: pannexin 1
• Calculated Molecular Weight: 426 aa, 48 kDa
• Observed Molecular Weight: 45-50 kDa, 90 kDa
• GenBank Accession Number: BC016931
• Gene Symbol: PANX1
• Gene ID (NCBI): 24145
• RRID: AB_2159186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PANX1, also named as MRS1, belongs to the pannexin family. It plays a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis. It is a structural component of the gap junctions and the hemichannels. PANX1 induced the formation of intercellular channels with characteristics of gap junctions. PANX1 is 48-kDa protein which contains 4 transmembrane domains and cytoplasmic N and C termini. It is a mediator of find-me signal/nucleotide release from apoptotic cells. Western blot for Panx1 was also seen in two bands at 45~50 kDa and ~90 kDa. The band of ~90 kDa corresponding to the Panx1 dimer size. (PMID: 24590064, PMID: 19009624)

Publications

Species	Application	Title
human	IHC	Theranostics; Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.; Authors - Qing Wang
human	WB	Sci Signal; Structures of human pannexin-1 in nanodiscs reveal gating mediated by dynamic movement of the N terminus and phospholipids.; Authors - Maki Kuzuya
human	WB	PLoS Pathog; Defining the proteolytic landscape during enterovirus infection.; Authors - Mohsan Saeed
rat	WB	Int J Mol Sci; Age-Dependent Activation of Pannexin1 Function Contributes to the Development of Epileptogenesis in Autosomal Dominant Sleep-related Hypermotor Epilepsy Model Rats; Authors - Kouji Fukuyama
human	IHC	Front Oncol; Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma.; Authors - Xiaojie Song
mouse	WB	Biochim Biophys Acta Mol Cell Res; TGF-β2 increases cell-cell communication in chondrocytes via p-Smad3 signalling.; Authors - Mengmeng Duan