PAPD5 Polyclonal antibody, PBS Only

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  WB analysis of HepG2 using 55197-1-AP (same clone as 55197-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55197-1-AP (PAPD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55197-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 55197-1-AP (same clone as 55197-1-PBS)

  IP result of anti-PAPD5 (IP:55197-1-AP, 3ug; Detection:55197-1-AP 1:500) with Jurkat cells lysate 1600ug. This data was developed using the same antibody clone with 55197-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PAPD5 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 55197-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

55197-1-PBS targets PAPD5 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PAP associated domain containing 5
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 63-80 kDa
• GenBank Accession Number: NM_001040285
• Gene Symbol: PAPD5
• Gene ID (NCBI): 64282
• RRID: AB_2881287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NDF8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.