- Featured Product
- KD/KO Validated
PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 14060-1-AP
Validation Data Gallery
|Positive WB detected in||C6 cells, HEK-293 cells, mouse brain tissue, SH-SY5Y cells, PC-12 cells, mouse liver tissue|
|Positive IHC detected in||mouse kidney tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, mouse brain tissue, mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14060-1-AP targets PARK2/Parkin in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, cattle, ducks, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARK2/Parkin fusion protein Ag5092|
|Full Name||Parkinson disease (autosomal recessive, juvenile) 2, parkin|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||42-52 kDa|
|GenBank accession number||BC022014|
|Gene ID (NCBI)||5071|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa.
Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.
Nucleic Acids Res
DNA damage invokes mitophagy through a pathway involving Spata18.
NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.
Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.
Proc Natl Acad Sci U S A
Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.
J Hazard Mater
Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ajte (Verified Customer) (03-08-2022)
It works well for me
Elyssa (Verified Customer) (11-11-2019)
This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.