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  • KD/KO Validated

PARK2/Parkin Polyclonal antibody

PARK2/Parkin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14060-1-AP

Synonyms

AR JP, LPRS2, PARK2, PARK2/Parkin, Parkin, Parkinson disease protein 2, PDJ, PRKN



Tested Applications

Positive WB detected inC6 cells, HEK-293 cells, mouse brain tissue, SH-SY5Y cells, PC-12 cells, mouse liver tissue
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, mouse brain tissue, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14060-1-AP targets PARK2/Parkin in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, cattle, ducks, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARK2/Parkin fusion protein Ag5092
Full Name Parkinson disease (autosomal recessive, juvenile) 2, parkin
Calculated molecular weight 52 kDa
Observed molecular weight 42-52 kDa
GenBank accession numberBC022014
Gene symbol PARK2
Gene ID (NCBI) 5071
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa.

Protocols

Product Specific Protocols
WB protocol for PARK2/Parkin antibody 14060-1-APDownload protocol
IHC protocol for PARK2/Parkin antibody 14060-1-APDownload protocol
IF protocol for PARK2/Parkin antibody 14060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
humanWB

Nucleic Acids Res

DNA damage invokes mitophagy through a pathway involving Spata18.

Authors - Xiuli Dan
humanWB

Dev Cell

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Authors - Oliver B Davis
mouseWB

Autophagy

Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.

Authors - Yinjuan Song
humanWB

Proc Natl Acad Sci U S A

Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.

Authors - Siddhesh Aras
ratWB,IF

J Hazard Mater

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Authors - Yiyuan Kang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ajte (Verified Customer) (03-08-2022)

It works well for me

  • Applications: Western Blot
FH

Elyssa (Verified Customer) (11-11-2019)

This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D)