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PARP1 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66520-1-Ig |CloneNo.: 1D7D4

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

PARP1 Antibody WB Jurkat cells 66520-1-Ig

Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours

PARP1 Antibody WB HSCT6 cells 66520-1-Ig

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours

PARP1 Antibody IP K-562 cells 66520-1-Ig

IP result of anti-PARP1(IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug

PARP1 Antibody IHC human lung cancer tissue 66520-1-Ig

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig( PARP1 antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARP1 Antibody IHC human lung cancer tissue 66520-1-Ig

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig( PARP1 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PARP1 Antibody IF HeLa cells 66520-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 66520-1-Ig (PARP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human, mouse, rat
Positive WB detected in:
Jurkat cells, RAW 264.7 cells, HeLa cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:100-1:1200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PARP1 fusion protein Ag19173
Full name:
poly (ADP-ribose) polymerase 1
Calculated molecular weight:
1014aa,113 kDa
Observed molecular weight:
113-116, 85-89 kDa
GenBank accession number:
BC037545
Gene ID (NCBI):
142
Gene symbol
PARP1
Synonyms
ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

Background

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kD. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.


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