PARP1 Monoclonal antibody
PARP1 Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
CloneNo.
1D7D4
Cat no : 66520-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, RAW 264.7 cells, HeLa cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:1200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 13 publications below |
Product Information
66520-1-Ig targets PARP1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, zebrafish |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PARP1 fusion protein Ag19173 |
Full Name | poly (ADP-ribose) polymerase 1 |
Calculated molecular weight | 1014 aa, 113 kDa |
Observed molecular weight | 113-116 kDa, 85-89 kDa |
GenBank accession number | BC037545 |
Gene symbol | PARP1 |
Gene ID (NCBI) | 142 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PARP1 antibody 66520-1-Ig | Download protocol |
IHC protocol for PARP1 antibody 66520-1-Ig | Download protocol |
IF protocol for PARP1 antibody 66520-1-Ig | Download protocol |
IP protocol for PARP1 antibody 66520-1-Ig | Download protocol |
FC protocol for PARP1 antibody 66520-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun SLC39A6/ZIP6 is essential for zinc homeostasis and T-cell development in zebrafish. | ||
Biochem Biophys Res Commun Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells. | ||
Oxid Med Cell Longev Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK. | ||
Exp Ther Med Poly(ADP-ribose) polymerase-1 inhibitor ameliorates dextran sulfate sodium-induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF-κB signaling pathway. | ||
Mol Med Rep Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. | ||
Chin Med Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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FH MANOHAR (Verified Customer) (12-11-2019) | No nonspecific binding
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