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PARP1 Monoclonal antibody

PARP1 Monoclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1D7D4

Cat no : 66520-1-Ig

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL



Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, RAW 264.7 cells, HeLa cells, ROS1728 cells, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, mouse colon tissue, mouse testis tissue, human breast cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1200
Immunofluorescence (IF)IF : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66520-1-Ig targets PARP1 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARP1 fusion protein Ag19173
Full Name poly (ADP-ribose) polymerase 1
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 85-89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2881883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
WB protocol for PARP1 antibody 66520-1-IgDownload protocol
IHC protocol for PARP1 antibody 66520-1-IgDownload protocol
IF protocol for PARP1 antibody 66520-1-IgDownload protocol
IP protocol for PARP1 antibody 66520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair

Authors - Liu Liu
ratWB,IP

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
humanWB

Biomaterials

Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.

Authors - Shaojun Pan
humanWB

Arthritis Rheumatol

Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.

Authors - Zhen Fan
humanWB

Theranostics

PMID: 31938062

Authors - Shuaishuai Zhang
humanWB

Cancer Lett

UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.

Authors - Dongying Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hadil (Verified Customer) (05-23-2023)

This antibody is a great choice, delivering strong signals and enhanced specificity. It has low background noise, ensuring accurate results. Its efficiency saves valuable time, making it a reliable and versatile tool for various applications. A must-have for researchers seeking reliable and efficient antibody performance.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: Mammalian cell lines
PARP1 Antibody Western Blot validation (1:10,000 dilution) in Mammalian cell lines (Cat no:66520-1-Ig)
FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other
FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T