PARP1 Monoclonal antibody

PARP1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D7D4

Cat no : 66520-1-Ig

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL



Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, HeLa cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

66520-1-Ig targets PARP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARP1 fusion protein Ag19173
Full Name poly (ADP-ribose) polymerase 1
Calculated molecular weight 1014 aa, 113 kDa
Observed molecular weight 113-116 kDa, 85-89 kDa
GenBank accession numberBC037545
Gene symbol PARP1
Gene ID (NCBI) 142
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
WB protocol for PARP1 antibody 66520-1-IgDownload protocol
IHC protocol for PARP1 antibody 66520-1-IgDownload protocol
IF protocol for PARP1 antibody 66520-1-IgDownload protocol
IP protocol for PARP1 antibody 66520-1-IgDownload protocol
FC protocol for PARP1 antibody 66520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Biochem Biophys Res Commun

SLC39A6/ZIP6 is essential for zinc homeostasis and T-cell development in zebrafish.

Authors - Lei Zhao
humanWB

Biochem Biophys Res Commun

Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.

Authors - Xiaoling Hong
humanWB

Chin Med

Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.

Authors - Hong-Yan Lin
humanWB

Am J Cancer Res

PMID: 29312803

Authors - Wanhu Zhu
humanWB

Acta Pharmacol Sin

PMID: 32300243

Authors - Xu Yao
humanWB

Theranostics

PMID: 31938062

Authors - Shuaishuai Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T