PAWR Polyclonal antibody, PBS Only

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  WB analysis of mouse heart using 20688-1-AP (same clone as 20688-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 20688-1-AP (same clone as 20688-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 20688-1-AP (same clone as 20688-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20688-1-AP (same clone as 20688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20688-1-AP (same clone as 20688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20688-1-AP (same clone as 20688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20688-1-AP (same clone as 20688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 20688-1-AP (same clone as 20688-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug PAWR Polyclonal antibody (20688-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

PAWR Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 20688-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

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Product Information

20688-1-PBS targets PAWR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PRKC, apoptosis, WT1, regulator
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_002583
• Gene Symbol: PAWR
• Gene ID (NCBI): 5074
• RRID: AB_10733473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96IZ0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAWR, also named as PAR4, is a pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. It induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. PAWR inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. It down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. PAWR may be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. The antibody is specific to PAWR.