PCMTD1 Polyclonal antibody, PBS Only

• 

  WB analysis using 24425-1-AP (same clone as 24425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24425-1-AP (same clone as 24425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24425-1-AP (same clone as 24425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24425-1-AP (same clone as 24425-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCMTD1 antibody (24425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24425-1-PBS in a different storage buffer formulation.

PCMTD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 24425-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

24425-1-PBS targets PCMTD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCMTD1 fusion protein Ag19634
• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1
• Calculated Molecular Weight: 357 aa, 41 kDa
• Observed Molecular Weight: 41 kDa, 27 kDa
• GenBank Accession Number: BC032670
• Gene Symbol: PCMTD1
• Gene ID (NCBI): 115294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96MG8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein-l-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1) is a putative E3 ubiquitin ligase substrate adaptor protein, which has a C-terminal domain containing SOCS-box ubiquitin ligase recruitment motifs. PCMTD1 protein may provide an alternate maintenance pathway for modified proteins in mammalian cells (PMID: 35486881). This antibody can detect two isoforms of PCMTD1, 27 kDa and 41 kDa respectively (PMID: 37953789, 35486881).