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- KD/KO Validated
PD-L1/CD274 (C-terminal) Polyclonal antibody
PD-L1/CD274 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 28076-1-AP
Validation Data Gallery
|Positive WB detected in
|IFN gamma treated A549 cells, mouse heart tissue, rat heart tissue, MDA-MB-231 cells, human placenta tissue, THP-1 cells
|Positive IP detected in
|human placenta tissue
|Positive IHC detected in
|human tonsillitis tissue, human placenta tissue, human breast cancer tissue, human lung cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human tonsillitis tissue
|Western Blot (WB)
|WB : 1:300-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
28076-1-AP targets PD-L1/CD274 (C-terminal) in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|PD-L1/CD274 (C-terminal) fusion protein Ag27557
|Calculated molecular weight
|290 aa, 33 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.
What is the molecular weight of PD-L1?
Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa).
What are the isoforms of PD-L1?
According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions.
What is the subcellular localization and tissue specificity of PD-L1?
It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues.
What is the function of PD-L1 in immune responses?
PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681).
How can PD-L1's implication in cancer be used as a drug target?
In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).
|Product Specific Protocols
|WB protocol for PD-L1/CD274 (C-terminal) antibody 28076-1-AP
|IHC protocol for PD-L1/CD274 (C-terminal) antibody 28076-1-AP
|IF protocol for PD-L1/CD274 (C-terminal) antibody 28076-1-AP
|IP protocol for PD-L1/CD274 (C-terminal) antibody 28076-1-AP
|Click here to view our Standard Protocols
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