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PD-L1/CD274 Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66248-1-Ig |CloneNo.: 2B11D11

Featured Product KD/KO validated

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Con: 232 μg/150 μl

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Tested Applications
Applications:
ELISA, FC, IF, IHC, WB
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Images See all

66248-1-Ig;HepG2 cells

HepG2, K-562, HSCT6, and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

66248-1-Ig;HepG2 cells

WB result of PD-L1 antibody (66248-1-Ig, 1:1000 and 1:2000) with si-Control and si-PDL1 transfected HepG2 cells.

66248-1-Ig;human tonsillitis tissue

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using ( PD-L1/CD274 Antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

66248-1-Ig;human tonsillitis tissue

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using ( PD-L1/CD274 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

66248-1-Ig;HeLa cells

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 66248-1-Ig(PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

66248-1-Ig;Raji cells

1X10^6 Raji cells were stained with 0.2ug PD-L1/CD274 antibody (66248-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, pig, rat
Positive WB detected in:
HepG2 cells, RAW 264.7 cells, HepG2 cells , human skeletal muscle tissue, pig lung tissue, human placenta tissue
Positive IHC detected in:
human tonsillitis tissue, mouse heart tissue, human lung cancer tissue, human placenta tissue, human heart tissue
Positive IF detected in:
HeLa cells
Positive FC detected in:
Raji cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:500
IF : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PD-L1/CD274 fusion protein Ag12443
Full name:
CD274 molecule
Calculated molecular weight:
290aa,33 kDa
Observed molecular weight:
50 kDa
GenBank accession number:
BC074984
Gene ID (NCBI):
29126
Gene symbol
CD274
Synonyms
B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1, PD-L1/CD274, Programmed death ligand 1
Background

Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation (PMID: 18760278; 16493058).


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