Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody

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  FC experiment of NIH/3T3 using 98256-1-RR

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody (98256-1-RR, Clone: 242025B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98256-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98256-1-RR against Mouse PDGFR alpha/CD140a were performed. The affinity constant is below 1 pM.

PDGFR alpha/CD140a Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98256-1-RR

Clone No.242025B3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

EC:2.7.10.1, CD140a, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor, Alpha platelet-derived growth factor receptor

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Tested Applications

• Positive FC detected in: NIH/3T3 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98256-1-RR targets PDGFR alpha/CD140a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: platelet derived growth factor receptor, alpha polypeptide
• Calculated Molecular Weight: 123kDa
• GenBank Accession Number: NM_001083316.2
• Gene Symbol: Pdgfra
• Gene ID (NCBI): 18595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P26618-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.