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Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody, PBS Only (98256-1-PBS)

Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody, PBS Only

Name: Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98256-1-PBS-1MG

PDGFR alpha/CD140a Uni-rAb^® Recombinant Antibody for FC

Cat No. 98256-1-PBS

Clone No. 242025B3

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

242025B3, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of NIH/3T3 using 98256-1-RR (same clone as 98256-1-PBS)
1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody (98256-1-RR, Clone: 242025B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98256-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody (98256-1-RR, Clone: 242025B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98256-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98256-1-RR
Biolayer interferometry (BLl) kinetic assays of 98256-1-RR against Mouse PDGFR alpha/CD140a were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
Product Information

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Product Information

98256-1-PBS targets PDGFR alpha/CD140a in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1625 Product name: Recombinant Mouse PDGF R alpha/PDGFRA protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 25-528 aa of NM_001083316.2 Sequence: LLLPSILPNENEKIVQLNSSFSLRCVGESEVSWQHPMSEEDDPNVEIRSEENNSGLFVTVLEVVNASAAHTGWYTCYYNHTQTDESEIEGRHIYIYVPDPDMAFVPLGMTDSLVIVEEDDSAIIPCRTTDPETQVTLHNNGRLVPASYDSRQGFNGTFSVGPYICEATVKGRTFKTSEFNVYALKATSELNLEMDARQTVYKAGETIVVTCAVFNNEVVDLQWTYPGEVRNKGITMLEEIKLPSIKLVYTLTVPKATVKDSGEYECAARQATKEVKEMKRVTISVHEKGFVEIEPTFGQLEAVNLHEVREFVVEVQAYPTPRISWLKDNLTLIENLTEITTDVQKSQETRYQSKLKLIRAKEEDSGHYTIIVQNEDDVKSYTFELSTLVPASILDLVDDHHGSGGGQTVRCTAEGTPLPEIDWMICKHIKKCNNDTSWTVLASNVSNIITELPRRGRSTVEGRVSFAKVEETIAVRCLAKNNLSVVARELKLVAPTLRSELTVA Predict reactive species
Full Name	platelet derived growth factor receptor, alpha polypeptide
Calculated Molecular Weight	123kDa
GenBank Accession Number	NM_001083316.2
Gene Symbol	Pdgfra
Gene ID (NCBI)	18595
RRID	AB_3745923
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P26618-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.