CoraLite® Plus 647 Anti-Mouse PDGFR beta/CD140b Rabbit Recombinant Antibody

• 

  FC experiment of NIH/3T3 using CL647-98207

  1x10^6 NIH/3T3 cells (left) or mouse splenocytes (right) were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse PDGFR beta/CD140b Rabbit RecAb (CL647-98207, Clone:241754F6) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

PDGFR beta/CD140b {ptg:Brand} Recombinant Antibody for FC

Cat No. CL647-98207

Clone No.241754F6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

EC:2.7.10.1, CD140b, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor, Beta platelet-derived growth factor receptor

---

Tested Applications

• Positive FC detected in: NIH/3T3 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98207 targets PDGFR beta/CD140b in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: platelet derived growth factor receptor, beta polypeptide
• Calculated Molecular Weight: 123kDa
• GenBank Accession Number: NM_008809.2
• Gene Symbol: PDGFR beta
• Gene ID (NCBI): 18596
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05622-1
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The Platelet-Derived Growth Factor Receptor Beta (PDGFRβ) is a receptor tyrosine kinase that plays a crucial role in various cellular processes, including cell proliferation, migration, and survival. In mice, PDGFRβ is particularly significant due to its expression in pericytes, which are cells that reside on the walls of capillaries and play a vital role in maintaining vascular stability and integrity (PMID: 20738866). In the central nervous system (CNS) of mice, PDGFRβ is predominantly expressed in pericytes and vascular smooth muscle cells. It is involved in the formation and maintenance of the blood-brain barrier, as well as in the regulation of angiogenesis and vascular stability. Research in mouse models has shown that PDGFRβ signaling is crucial for tissue repair and functional recovery after stroke. Mice with disrupted PDGFRβ signaling exhibit delayed recovery and increased infarction volume following cerebral ischemia (PMID: 21952111). The observed MW is about 180 kDa, which may be caused by post-translational modification.