PDGFR beta/CD140b Polyclonal antibody

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  WB analysis using 13449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13449-1-AP

  IP result of anti-PDGFR beta (IP:13449-1-AP, 4ug; Detection:13449-1-AP 1:5000) with mouse lung tissue lysate 1120 ug.

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  IHC staining of human placenta using 13449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

PDGFR beta/CD140b Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13449-1-AP

Publications(46)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 35 publications below
• IHC: See 6 publications below
• IP: See 1 publications below

Product Information

13449-1-AP targets PDGFR beta/CD140b in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGFR beta/CD140b fusion protein Ag4285
• Full Name: platelet-derived growth factor receptor, beta polypeptide
• Calculated Molecular Weight: 1106 aa, 124 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC032224
• Gene Symbol: PDGFR beta
• Gene ID (NCBI): 5159
• RRID: AB_2162644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09619
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

Publications

Species	Application	Title
rat	WB	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv ; KD Validated
human		Cell Rep; Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells; Authors - Hiraku Tsujimoto
mouse	WB	J Mol Cell Biol; BMP4 mediates the interplay between adipogenesis and angiogenesis during expansion of subcutaneous white adipose tissue.; Authors - Yan Tang
		Br J Cancer; Inhibition of PFKFB3 in HER2-positive gastric cancer improves sensitivity to trastuzumab by inducing tumour vessel normalisation.; Authors - Xingxing Yao
rat		Neurotherapeutics; A switch in the pathway of TRPC3-mediated calcium influx into brain pericytes contributes to capillary spasms after subarachnoid hemorrhage; Authors - Yuncong Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other