PDGFR beta/CD140b Polyclonal antibody, PBS Only

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  WB analysis using 13449-1-AP (same clone as 13449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 13449-1-AP (same clone as 13449-1-PBS)

  IP result of anti-PDGFR beta (IP:13449-1-AP, 4ug; Detection:13449-1-AP 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 13449-1-AP (same clone as 13449-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13449-1-AP (same clone as 13449-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PDGFR beta/CD140b Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13449-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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Product Information

13449-1-PBS targets PDGFR beta/CD140b in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGFR beta/CD140b fusion protein Ag4285
• Full Name: platelet-derived growth factor receptor, beta polypeptide
• Calculated Molecular Weight: 1106 aa, 124 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC032224
• Gene Symbol: PDGFR beta
• Gene ID (NCBI): 5159
• RRID: AB_2162644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09619
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)