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PDI Polyclonal antibody
PDI Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 11245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COLO 320 cells, HEK-293 cells, HepG2 cells, mouse heart tissue, rat brain tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 35 publications below |
IHC | See 11 publications below |
IF | See 19 publications below |
FC | See 1 publications below |
Product Information
11245-1-AP targets PDI in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
Tested Reactivity | human, mouse, rat, zebrafish |
Cited Reactivity | human, mouse, rat, pig, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PDI fusion protein Ag1747 |
Full Name | prolyl 4-hydroxylase, beta polypeptide |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 57 kDa |
GenBank Accession Number | BC014504 |
Gene Symbol | PDI |
Gene ID (NCBI) | 5034 |
RRID | AB_2298937 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PDI antibody 11245-1-AP | Download protocol |
IHC protocol for PDI antibody 11245-1-AP | Download protocol |
IF protocol for PDI antibody 11245-1-AP | Download protocol |
IP protocol for PDI antibody 11245-1-AP | Download protocol |
FC protocol for PDI antibody 11245-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Thromb Haemost VWF-Gly2752Ser, a novel non-cysteine substitution variant in the CK domain, exhibits severe secretory impairment by hampering C-terminal dimer formation. | ||
Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Sci Adv Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (08-19-2024) | Worked well at 1:2000
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FH Anastasia (Verified Customer) (08-07-2024) |
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