ERp57/ERp60 Polyclonal antibody

• Featured Product
• KD/KO Validated

ERp57/ERp60 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 15967-1-AP

Synonyms

58 kDa microsomal protein, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER protein 57, ER protein 60, ER60, ERp57, ERp57/ERp60, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

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Validation Data Gallery

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  WB analysis using 15967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15967-1-AP

  IP Result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung cancer using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (15967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 15967-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ERp57/ERp60 antibody (15967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: A375 cells, L02 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

15967-1-AP targets ERp57/ERp60 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERp57/ERp60 fusion protein Ag8741
• Full Name: protein disulfide isomerase family A, member 3
• Calculated molecular weight: 505 aa, 57 kDa
• Observed molecular weight: 57 kDa
• GenBank accession number: BC014433
• Gene symbol: PDIA3
• Gene ID (NCBI): 2923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Publications

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
pig	WB	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
mouse	WB	Redox Biol; Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress.; Authors - Xie Ge
rat	IF	EMBO J; TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization.; Authors - Adam Johnson
human	IF	Cell Rep; Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.; Authors - Jeffrey R Jones
human	IF	J Clin Endocrinol Metab; Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.; Authors - Liu Xin-Mei XM

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Prasanna (Verified Customer) (05-07-2018)

Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: normal rat kidney cells