• Featured Product
  • KD/KO Validated

ERp57/ERp60 Polyclonal antibody

ERp57/ERp60 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15967-1-AP

Synonyms

58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER protein 57, ER protein 60, ER60, ERp57, ERp57/ERp60, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3



Tested Applications

Positive WB detected inA375 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15967-1-AP targets ERp57/ERp60 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp57/ERp60 fusion protein Ag8741
Full Name protein disulfide isomerase family A, member 3
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014433
Gene Symbol PDIA3
Gene ID (NCBI) 2923
RRIDAB_2236784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protocols

Product Specific Protocols
WB protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
IHC protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
IF protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
IP protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
pigWB

Mol Biol Evol

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.

Authors - Ke Chen
mouseWB

Redox Biol

Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress.

Authors - Xie Ge
ratIF

EMBO J

TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization.

Authors - Adam Johnson
humanIF

Cell Rep

Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.

Authors - Jeffrey R Jones
humanIF

J Clin Endocrinol Metab

Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.

Authors - Liu Xin-Mei XM

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Prasanna (Verified Customer) (05-07-2018)

Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: normal rat kidney cells