Various lysates were subjected to SDS PAGE followed by western blot with 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours

A375 cells were subjected to SDS PAGE followed by western blot with 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

L02 cells were subjected to SDS PAGE followed by western blot with 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

L02 cells were subjected to SDS PAGE followed by western blot with 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

rat liver tissue were subjected to SDS PAGE followed by western blot with 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP( ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP( ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of HepG2 cells, using PDIA3 antibody 15967-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 HepG2 cells were stained with 0.2ug ERp57/ERp60 antibody (15967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.