ERp57/ERp60 Polyclonal antibody

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  WB analysis using 15967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15967-1-AP

  IP result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung cancer using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (15967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

ERp57/ERp60 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15967-1-AP

Publications(43)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ERp57, PDIA3, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60, EC:5.3.4.1

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Tested Applications

• Positive WB detected in: A375 cells, L02 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

15967-1-AP targets ERp57/ERp60 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERp57/ERp60 fusion protein Ag8741
• Full Name: protein disulfide isomerase family A, member 3
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014433
• Gene Symbol: PDIA3
• Gene ID (NCBI): 2923
• RRID: AB_2236784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30101
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
mouse	WB,IHC	J Control Release; A facile and smart strategy to enhance bone regeneration with efficient vitamin D3 delivery through sterosome technology; Authors - Shuxi Ye
pig	WB	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
mouse	WB	Redox Biol; Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress.; Authors - Xie Ge
rat	IF	EMBO J; TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization.; Authors - Adam Johnson
human	IF	Cell Rep; Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.; Authors - Jeffrey R Jones

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Prasanna (Verified Customer) (05-07-2018)

Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: normal rat kidney cells