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  • KD/KO Validated

ERp57/ERp60 Polyclonal antibody

ERp57/ERp60 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15967-1-AP

Synonyms

58 kDa microsomal protein, Disulfide isomerase ER 60, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3



Tested Applications

Positive WB detected inA375 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15967-1-AP targets ERp57/ERp60 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp57/ERp60 fusion protein Ag8741
Full Name protein disulfide isomerase family A, member 3
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014433
Gene symbol PDIA3
Gene ID (NCBI) 2923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protocols

Product Specific Protocols
WB protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
IHC protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
IF protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
IP protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
FC protocol for ERp57/ERp60 antibody 15967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, ratWB,IHC

Sci China Life Sci

Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.

Authors - Qianqian Zhao
humanIHC

Oncotarget

Combination of CALR and PDIA3 is a potential prognostic biomarker for non-small cell lung cancer.

Authors - Ke Wang
IHC

Onco Targets Ther

Identification of PGAM1 as a putative therapeutic target for pancreatic ductal adenocarcinoma metastasis using quantitative proteomics.

Authors - Xinlu Liu
humanWB

Graefes Arch Clin Exp Ophthalmol

Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.

Authors - Cheng Kai-Chun KC
humanWB,IHC

Tumour Biol

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Authors - Ying Zhu
  • KD Validated
ratWB

Chem Res Toxicol

Effect of Subcellular Translocation of Protein Disulfide Isomerase on Tetrachlorobenzoquinone-induced Signaling Shift from Endoplasmic Reticulum Stress to Apoptosis.

Authors - Zixuan Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Prasanna (Verified Customer) (05-07-2018)

Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: normal rat kidney cells