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ERp57/ERp60 Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 15967-1-AP

Featured Product KD/KO validated

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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ERp57/ERp60 Antibody IF HepG2 cells 15967-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ERp57/ERp60 Antibody WB L02 cells 15967-1-AP

L02 cells were subjected to SDS PAGE followed by western blot with 15967-1-AP(ERp57/ERp60 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ERp57/ERp60 Antibody IHC human lung cancer tissue 15967-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP( ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ERp57/ERp60 Antibody IHC human lung cancer tissue 15967-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP( ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ERp57/ERp60 Antibody IP mouse liver tissue 15967-1-AP

IP Result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

ERp57/ERp60 Antibody FC HepG2 cells 15967-1-AP

1X10^6 HepG2 cells were stained with 0.2ug ERp57/ERp60 antibody (15967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
L02 cells, A375 cells, mouse liver tissue, rat liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2400
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ERp57/ERp60 fusion protein Ag8741
Full name:
protein disulfide isomerase family A, member 3
Calculated molecular weight:
505aa,57 kDa
Observed molecular weight:
57 kDa
GenBank accession number:
BC014433
Gene ID (NCBI):
2923
Gene symbol
PDIA3
Synonyms
58 kDa microsomal protein, Disulfide isomerase ER 60, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

Background

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ERp57/ERp60 Rabbit Polyclonal antibody

Prasanna (Verified Customer), 05-07-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1000
Secondary Antibody Dilution
1:10,000
No image submitted with review

No image submitted with review

Cell/Tissue Type
normal rat kidney cells
Lot Number
00049162
Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.

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