PDIA3 Recombinant antibody

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  WB analysis using 85087-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85087-5-RR (PDIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 85087-5-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85087-5-RR (PDIA3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 85087-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDIA3 Recombinant antibody (85087-5-RR, Clone:242735H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85087-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85087-5-RR against Human PDIA3 were performed. The affinity constant is 13.0 nM.

PDIA3 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 85087-5-RR

Clone No.242735H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

ERp57, 242735H7, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60, EC:5.3.4.1

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85087-5-RR targets PDIA3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDIA3 fusion protein Ag8741
• Full Name: protein disulfide isomerase family A, member 3
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014433
• Gene Symbol: PDIA3
• Gene ID (NCBI): 2923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30101
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.