PDIA3 Recombinant antibody, PBS Only

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  WB analysis using 85087-5-RR (same clone as 85087-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85087-5-RR (PDIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85087-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 85087-5-RR (same clone as 85087-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85087-5-RR (PDIA3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85087-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85087-5-RR (same clone as 85087-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDIA3 Recombinant antibody (85087-5-RR, Clone:242735H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85087-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85087-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85087-5-RR against Human PDIA3 were performed. The affinity constant is 13.0 nM.

PDIA3 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 85087-5-PBS

Clone No.242735H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

ERp57, 242735H7, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60, EC:5.3.4.1

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Product Information

85087-5-PBS targets PDIA3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDIA3 fusion protein Ag8741
• Full Name: protein disulfide isomerase family A, member 3
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014433
• Gene Symbol: PDIA3
• Gene ID (NCBI): 2923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30101
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.