Podoplanin Polyclonal antibody

Podoplanin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 11629-1-AP


AGGRUS, D2 40, D2 40,M2A, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11629-1-AP targets Podoplanin in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Podoplanin fusion protein Ag2201
Full Name podoplanin
Calculated Molecular Weight 17 kDa, 25 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC022812
Gene Symbol Podoplanin
Gene ID (NCBI) 10630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podoplanin was identified as a 43 kDa glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.


Product Specific Protocols
WB protocol for Podoplanin antibody 11629-1-APDownload protocol
IHC protocol for Podoplanin antibody 11629-1-APDownload protocol
FC protocol for Podoplanin antibody 11629-1-APDownload protocol
Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Amelia (Verified Customer) (07-22-2022)

PDPN was used for mass cytometry, conjugated in-house.

  • Applications: Other