PGAM2 Polyclonal antibody

PGAM2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15550-1-AP

Synonyms

BPG dependent PGAM 2, PGAM M, PGAM2, PGAMM, Phosphoglycerate mutase 2



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, mouse skeletal muscle tissue, rat heart tisssue, rat skeletal muscle tissue
Positive IHC detected inhuman breast cancer tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15550-1-AP targets PGAM2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGAM2 fusion protein Ag7908
Full Name phosphoglycerate mutase 2 (muscle)
Calculated molecular weight 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC001904
Gene symbol PGAM2
Gene ID (NCBI) 5224
RRIDAB_2299336
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PGAM2 antibody 15550-1-APDownload protocol
IHC protocol for PGAM2 antibody 15550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

EMBO Rep

METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells

Authors - Yichao Hou
pigWB

Sci Rep

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Authors - Liangliang Fu
humanWB

J Proteomics

Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility.

Authors - Yueshuai Guo
mouseWB

Sci Adv

Lactate metabolism is essential in early-onset mitochondrial myopathy

Authors - Zhenkang Chen
mouseWB

Redox Biol

Unbalanced redox status network as an early pathological event in congenital cataracts

Authors - Eloy Bejarano
human,mouseWB,IP

Cell Res

Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate

Authors - Yu-Qing Wu