PGAM2 Polyclonal antibody

PGAM2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 15550-1-AP

Synonyms

BPG dependent PGAM 2, PGAM M, PGAM2, PGAMM, Phosphoglycerate mutase 2

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Validation Data Gallery

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  WB analysis using 15550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15550-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15550-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15550-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15550-1-AP (PGAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15550-1-AP (PGAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse skeletal muscle tissue, rat heart tisssue, rat skeletal muscle tissue
• Positive IHC detected in: human breast cancer tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15550-1-AP targets PGAM2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGAM2 fusion protein Ag7908
• Full Name: phosphoglycerate mutase 2 (muscle)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC001904
• Gene Symbol: PGAM2
• Gene ID (NCBI): 5224
• RRID: AB_2299336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.

Publications

Species	Application	Title
human,mouse	IHC,IF	EMBO Rep; METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells; Authors - Yichao Hou
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	WB	J Proteomics; Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility.; Authors - Yueshuai Guo
mouse	WB	Sci Adv; Lactate metabolism is essential in early-onset mitochondrial myopathy; Authors - Zhenkang Chen
mouse	WB	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano
human,mouse	WB,IP	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-04-2024)

A reliable antibody for westernblots

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: NRVCMs