PGAP1 Polyclonal antibody, PBS Only

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  WB analysis of human testis using 55392-1-AP (same clone as 55392-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 55392-1-AP (same clone as 55392-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55392-1-AP (same clone as 55392-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 55392-1-AP (same clone as 55392-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 55392-1-AP (same clone as 55392-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 55392-1-AP (same clone as 55392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 55392-1-AP (same clone as 55392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55392-1-AP (same clone as 55392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55392-1-AP (same clone as 55392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 55392-1-AP (same clone as 55392-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PGAP1 antibody (55392-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55392-1-PBS in a different storage buffer formulation.

PGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55392-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

55392-1-PBS targets PGAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: post-GPI attachment to proteins 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 49-53 kDa, 85-90 kDa
• GenBank Accession Number: NM_024989
• Gene Symbol: PGAP1
• Gene ID (NCBI): 80055
• RRID: AB_11232412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q75T13
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.