PI16 Polyclonal antibody, PBS Only

• 

  WB analysis using 30527-1-AP (same clone as 30527-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30527-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30527-1-AP (same clone as 30527-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SKOV-3 using 30527-1-AP (same clone as 30527-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30527-1-PBS in a different storage buffer formulation.

PI16 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 30527-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

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Product Information

30527-1-PBS targets PI16 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI16 fusion protein Ag32556
• Full Name: peptidase inhibitor 16
• Calculated Molecular Weight: 463 aa, 49 kDa
• Observed Molecular Weight: 100 kDa, 49 kDa
• GenBank Accession Number: BC022399
• Gene Symbol: PI16
• Gene ID (NCBI): 221476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UXB8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).