PIM1 Polyclonal antibody

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  WB analysis using 27196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27196-1-AP (PIM1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PIM1 Polyclonal Antibody for WB, ELISA

Cat No. 27196-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PIM, pim 1 oncogene

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27196-1-AP targets PIM1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIM1 fusion protein Ag25743
• Full Name: pim-1 oncogene
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC020224
• Gene Symbol: PIM1
• Gene ID (NCBI): 5292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11309
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIM1 belongs to the Ser/Thr protein kinase family and the Pim subfamily. This family includes PIM1, PIM2, and PIM3, which share high sequence and structural similarity. PIM1 is a serine/threonine protein kinase that significantly promotes cell survival and proliferation, providing a selective advantage for tumorigenesis. PIM1 is expressed primarily in cells of the hematopoietic and germline lineages (PMID: 16186805).