• Featured Product
  • KD/KO Validated

PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10495-1-AP


DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, NIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10495-1-AP targets PIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIN1 fusion protein Ag0767
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002899
Gene symbol PIN1
Gene ID (NCBI) 5300
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).


Product Specific Protocols
WB protocol for PIN1 antibody 10495-1-APDownload protocol
IHC protocol for PIN1 antibody 10495-1-APDownload protocol
IF protocol for PIN1 antibody 10495-1-APDownload protocol
IP protocol for PIN1 antibody 10495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Pineal Res

Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.

Authors - Dongmei Chen

J Hazard Mater

Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.

Authors - Fuli Zheng


SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Authors - Ling Lin

Nucleic Acids Res

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

Authors - Poolman Toryn M TM
  • KD Validated
mouseWB, IHC, IF

Oxid Med Cell Longev

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.

Authors - Yanzhuo Zhang

Regen Biomater

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways

Authors - Fengzhu Zhang
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other