PITRM1 Polyclonal antibody

PITRM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10101-2-AP

Synonyms

MP1, Metalloprotease 1, KIAA1104, hPreP, hMP1

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Validation Data Gallery

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  WB analysis of human placenta using 10101-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10101-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10101-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10101-2-AP

  IP result of anti-PITRM1 (IP:10101-2-AP, 4ug; Detection:10101-2-AP 1:500) with A549 cells lysate 2000ug.

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  IHC staining of human breast cancer using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 10101-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PITRM1 antibody (10101-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: human placenta tissue, A549 cells, mouse testis tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue, human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10101-2-AP targets PITRM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PITRM1 fusion protein Ag0150
• Full Name: pitrilysin metallopeptidase 1
• Calculated Molecular Weight: 60 kDa, 117 kDa
• Observed Molecular Weight: 110-117 kDa
• GenBank Accession Number: BC001150
• Gene Symbol: PITRM1
• Gene ID (NCBI): 10531
• RRID: AB_2165145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.

Publications

Species	Application	Title
human	WB	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
mouse	WB	PLoS One; Proteolysis controls endogenous substance p levels.; Authors - Mitchell Andrew J AJ