Validation Data Gallery
|Positive WB detected in
|human placenta tissue, A549 cells, mouse testis tissue
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human osteosarcoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:200-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:100-1:400
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10101-2-AP targets PITRM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|PITRM1 fusion protein Ag0150
|pitrilysin metallopeptidase 1
|Calculated molecular weight
|60 kDa, 117 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.
|Product Specific Protocols
|WB protocol for PITRM1 antibody 10101-2-AP
|IHC protocol for PITRM1 antibody 10101-2-AP
|IF protocol for PITRM1 antibody 10101-2-AP
|IP protocol for PITRM1 antibody 10101-2-AP
|Click here to view our Standard Protocols