tPA Polyclonal antibody

tPA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10147-1-AP

Synonyms

Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA



Tested Applications

Positive WB detected inmouse pancreas tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue, human ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10147-1-AP targets tPA in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen tPA fusion protein Ag0200
Full Name plasminogen activator, tissue
Calculated molecular weight 57 kDa
Observed molecular weight 32-35 kDa, 65 kDa
GenBank accession numberBC002795
Gene symbol PLAT
Gene ID (NCBI) 5327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Protocols

Product Specific Protocols
WB protocol for tPA antibody 10147-1-APDownload protocol
IHC protocol for tPA antibody 10147-1-APDownload protocol
IF protocol for tPA antibody 10147-1-APDownload protocol
IP protocol for tPA antibody 10147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Cycle

tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.

Authors - Ling Chen
ratWB, IF

Neuropsychiatr Dis Treat

Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway.

Authors - Hao Dong
humanWB

Aging (Albany NY)

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Authors - Pingping Ma
ratWB

Biosci Rep

2,5-Hexanedione induced apoptosis in rat spinal cord neurons and VSC4.1 cells via the proNGF/p75NTR and JNK pathways.

Authors - Mengxin Luo
humanWB

J Proteomics

Bottom up proteomics reveals novel differentiation proteins in neuroblastoma cells treated with 13-cis retinoic acid.

Authors - Effie G Halakos
humanWB

Int J Biol Macromol

Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.

Authors - Dongsheng Sun