PLEKHH2 Polyclonal antibody

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  WB analysis of Jurkat using 14204-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14204-1-AP (PLEKHH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PLEKHH2 Polyclonal Antibody for WB, ELISA

Cat No. 14204-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

KIAA2028, PLEKHH1L, PLEKHH2

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14204-1-AP targets PLEKHH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEKHH2 fusion protein Ag5409
• Full Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 2
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC063310
• Gene Symbol: PLEKHH2
• Gene ID (NCBI): 130271
• RRID: AB_2878027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVE3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEKHH2 is a 1491-residue intracellular protein highly enriched in renal glomerular podocytes. PLEKHH2 is one of two previously uncharacterized PLEKHH proteins encoded in the mammalian genomes. A PLEKHH ortholog has also been described in Drosophila, Caenorhabditis elegans, and zebrafish. In C. elegans, the loss of the protein was found to cause variable axon guidance defects.

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; PLEKHH2 binds β-arrestin1 through its FERM domain, activates FAK/PI3K/AKT phosphorylation, and promotes the malignant phenotype of non-small cell lung cancer; Authors - Rui Wang