- Featured Product
- KD/KO Validated
Podocalyxin Polyclonal antibody
Podocalyxin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, IF, FC, ELISA
Cat no : 18150-1-AP
Validation Data Gallery
|Positive WB detected in||human kidney tissue, HEK-293 cells, HepG2 cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human kidney tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:4000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 6 publications below|
|IF||See 2 publications below|
|FC||See 1 publications below|
The immunogen of 18150-1-AP is Podocalyxin Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Podocalyxin fusion protein Ag12844|
|Calculated molecular weight||526 aa, 55 kDa|
|Observed molecular weight||60-70 kDa|
|GenBank accession number||BC093730|
|Gene ID (NCBI)||5420|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).
|Product Specific Protocols|
|WB protocol for Podocalyxin antibody 18150-1-AP||Download protocol|
|IHC protocol for Podocalyxin antibody 18150-1-AP||Download protocol|
|IF protocol for Podocalyxin antibody 18150-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis.
Urinary extracellular vesicles and micro-RNA as markers of acute kidney injury after cardiac surgery.
Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.
Int J Cardiol
Brg1 deficiency in vascular endothelial cells blocks neutrophil recruitment and ameliorates cardiac ischemia-reperfusion injury in mice.
Clin Sci (Lond)
First identification of PODXL nonsense mutations in autosomal dominant focal segmental glomerulosclerosis.
Int J Mol Sci
Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.