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- KD/KO Validated
Podocalyxin Polyclonal antibody
Podocalyxin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18150-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human kidney tissue, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human kidney tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:4000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IF | See 3 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 18150-1-AP is Podocalyxin Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Podocalyxin fusion protein Ag12844 |
| Full Name | podocalyxin-like |
| Calculated Molecular Weight | 526 aa, 55 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC093730 |
| Gene Symbol | PODXL |
| Gene ID (NCBI) | 5420 |
| RRID | AB_11044193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Podocalyxin antibody 18150-1-AP | Download protocol |
| IHC protocol for Podocalyxin antibody 18150-1-AP | Download protocol |
| IF protocol for Podocalyxin antibody 18150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Tissue Eng Part A Genome engineering of human urine-derived stem cells to express lactoferrin and deoxyribonuclease | ||
Int J Cardiol Brg1 deficiency in vascular endothelial cells blocks neutrophil recruitment and ameliorates cardiac ischemia-reperfusion injury in mice. | ||
Sci Rep Urinary extracellular vesicles and micro-RNA as markers of acute kidney injury after cardiac surgery. | ||
STAR Protoc Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells. |
