Podocalyxin Polyclonal antibody

• Featured Product
• KD/KO Validated

Podocalyxin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 18150-1-AP

Synonyms

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, Renal Cell Carcinoma marker, TRA 1 81,TRA 1 60, TRA-1-60

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Validation Data Gallery

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  WB analysis of HEK-293 using 18150-1-AP

  WB result of Podocalyxin antibody (18150-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Podocalyxin transfected HEK-293 cells.

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  WB analysis of human kidney using 18150-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18150-1-AP (PODXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18150-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18150-1-AP (PODXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18150-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18150-1-AP (PODXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18150-1-AP (Podocalyxin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18150-1-AP (Podocalyxin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 18150-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (18150-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9, red).

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  FC experiment of HeLa using 18150-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Podocalyxin antibody (18150-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: human kidney tissue, HEK-293 cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human kidney tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Product Information

The immunogen of 18150-1-AP is Podocalyxin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Podocalyxin fusion protein Ag12844
• Full Name: podocalyxin-like
• Calculated molecular weight: 526 aa, 55 kDa
• Observed molecular weight: 60-70 kDa
• GenBank accession number: BC093730
• Gene symbol: PODXL
• Gene ID (NCBI): 5420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

Publications

Species	Application	Title
human	WB	Sci Rep; Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis.; Authors - Akiko Tanoue
human	FC	Sci Rep; Urinary extracellular vesicles and micro-RNA as markers of acute kidney injury after cardiac surgery.; Authors - Douglas Miller
human	IF	STAR Protoc; Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.; Authors - Julie Bejoy
mouse	WB	Int J Cardiol; Brg1 deficiency in vascular endothelial cells blocks neutrophil recruitment and ameliorates cardiac ischemia-reperfusion injury in mice.; Authors - Xinjian Zhang
human	WB	Clin Sci (Lond); First identification of PODXL nonsense mutations in autosomal dominant focal segmental glomerulosclerosis.; Authors - Fu-Jun Lin ; KD Validated
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani

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