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Podocalyxin Polyclonal antibody

Podocalyxin Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 18150-1-AP


GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, Renal Cell Carcinoma marker, TRA 1 81,TRA 1 60, TRA-1-60

Tested Applications

Positive WB detected inhuman kidney tissue, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18150-1-AP is Podocalyxin Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Podocalyxin fusion protein Ag12844
Full Name podocalyxin-like
Calculated Molecular Weight 526 aa, 55 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC093730
Gene Symbol PODXL
Gene ID (NCBI) 5420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).


Product Specific Protocols
WB protocol for Podocalyxin antibody 18150-1-APDownload protocol
IHC protocol for Podocalyxin antibody 18150-1-APDownload protocol
IF protocol for Podocalyxin antibody 18150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis.

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Urinary extracellular vesicles and micro-RNA as markers of acute kidney injury after cardiac surgery.

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