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Cyclophilin B Polyclonal antibody
Cyclophilin B Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11607-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, U-937 cells, Raji cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, rat liver tissue |
Positive IHC detected in | human lymphoma tissue, human liver cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 11 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
Product Information
11607-1-AP targets Cyclophilin B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cyclophilin B fusion protein Ag2161 |
Full Name | peptidylprolyl isomerase B (cyclophilin B) |
Calculated molecular weight | 216 aa, 24 kDa |
Observed molecular weight | 19-24 kDa |
GenBank accession number | BC032138 |
Gene symbol | PPIB |
Gene ID (NCBI) | 5479 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for Cyclophilin B antibody 11607-1-AP | Download protocol |
IHC protocol for Cyclophilin B antibody 11607-1-AP | Download protocol |
IF protocol for Cyclophilin B antibody 11607-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Prolif OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy | ||
Hum Mol Genet Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes. | ||
Cells HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy | ||
J Virol CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. |