Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, U-937 cells, Jurkat cells, Raji cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human normal colon, human liver cancer tissue, human lymphoma tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11607-1-AP targets Cyclophilin B in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2161 Product name: Recombinant human PPIB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC032138 Sequence: MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE Predict reactive species |
| Full Name | peptidylprolyl isomerase B (cyclophilin B) |
| Calculated Molecular Weight | 216 aa, 24 kDa |
| Observed Molecular Weight | 19-24 kDa |
| GenBank Accession Number | BC032138 |
| Gene Symbol | Cyclophilin B |
| Gene ID (NCBI) | 5479 |
| RRID | AB_2237519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23284 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclophilin B (CYPB) , also named as PPIB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cyclophilin B antibody 11607-1-AP | Download protocol |
| IHC protocol for Cyclophilin B antibody 11607-1-AP | Download protocol |
| WB protocol for Cyclophilin B antibody 11607-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Cyclophilin B Polyclonal antibody (Cat# 11607-1-AP) has 25 citations across journals including Molecular Cell, Cell Reports, American Journal of Human Genetics, Journal of Translational Medicine, Cell Proliferation, International Immunopharmacology, Human Molecular Genetics, and Proteomics. Research fields span cancer biology (lung, ovarian, colorectal), renal injury and diabetic nephropathy, osteogenesis imperfecta, mitochondrial dynamics, autophagy, viral infections, COPD, hair follicle stem cells, and protein quality control. Applications are predominantly Western blot, with IHC, IF, and RIP also represented.
| Species | Application | Title |
|---|---|---|
Biomater Res Ultrasound-Targeted Nanobubbles Codelivering NKP-1339 and miR-142-5p for Synergistic Mitochondrial Immunogenic Cell Death and PD-L1 Inhibition in Cancer Therapy. | ||
J Transl Med LINC02888 promotes HGSOC progression and immune evasion via PPIB-mediated stabilization of LAPTM5 mRNA and inhibition of RIG-I-like receptor signaling
| ||
Cell Rep USP8 deubiquitylation-boosted NSUN4 potentiates lung squamous cell carcinoma progression by m5C methylation of DHCR24. | ||
Cell Prolif OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. One user employed it as a loading control in HAP1 cells at a 1:5000 dilution, reporting a strong and rapid signal on film with standard ECL substrate. The other user, also at 1:5000 dilution, simply noted that it "works well." Overall, the reviews reflect consistent satisfaction with the antibody's performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ganesh (Verified Customer) (02-07-2024) | Whole cell extracts of HAP1 (human) cells were resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. Membrane was incubated overnight at 4 deg C with cyclophilin antibody (diluted in 5% Milk in TBS-T). I used this antibody as a loading control in this experiment. The signal was very quick on a film using regular ECL substrate.
 |
Parijat (Verified Customer) (08-21-2023) | Works well.
|
