Cyclophilin B Polyclonal antibody

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  WB analysis of HeLa using 11607-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11607-1-AP

  WB result of Cyclophilin B antibody (11607-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.

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  WB analysis of Raji using 11607-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 11607-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11607-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11607-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11607-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11607-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11607-1-AP (Cyclophilin B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11607-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11607-1-AP

  Immunofluorescent analysis of HeLa cells using 11607-1-AP (Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Cyclophilin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11607-1-AP

Publications(16)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PPIB, PPIase B, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1

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Tested Applications

• Positive WB detected in: HeLa cells, U-937 cells, Raji cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, rat liver tissue
• Positive IHC detected in: human normal colon, human liver cancer tissue, human lymphoma tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

11607-1-AP targets Cyclophilin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclophilin B fusion protein Ag2161
• Full Name: peptidylprolyl isomerase B (cyclophilin B)
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 19-24 kDa
• GenBank Accession Number: BC032138
• Gene Symbol: Cyclophilin B
• Gene ID (NCBI): 5479
• RRID: AB_2237519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23284
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).

Publications

Species	Application	Title
human	WB	Mol Cell; Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells.; Authors - Pei-Hsuan Chen
human	WB,IHC	Am J Hum Genet; PPIB mutations cause severe osteogenesis imperfecta.; Authors - van Dijk Fleur S FS
mouse	WB	Cell Prolif; OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy; Authors - Fan Zhao
human	WB,IF	Hum Mol Genet; Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes.; Authors - Pyott Shawna M SM
mouse	WB	Cells; HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy; Authors - Lang Shi
human,mouse	WB,IF	J Virol; CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.; Authors - Watanabe Akira A

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ganesh (Verified Customer) (02-07-2024)

Whole cell extracts of HAP1 (human) cells were resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. Membrane was incubated overnight at 4 deg C with cyclophilin antibody (diluted in 5% Milk in TBS-T). I used this antibody as a loading control in this experiment. The signal was very quick on a film using regular ECL substrate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000 dilution
• Cell Tissue Type: HAP1

Cyclophilin B Antibody Western Blot validation (1:5000 dilution dilution) in HAP1 (Cat no:11607-1-AP)

FH

Parijat (Verified Customer) (08-21-2023)

Works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000