• Featured Product
  • KD/KO Validated

Cyclophilin B Polyclonal antibody

Cyclophilin B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11607-1-AP

Synonyms

Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP



Tested Applications

Positive WB detected inHeLa cells, U-937 cells, Raji cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, rat liver tissue
Positive IHC detected inhuman lymphoma tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11607-1-AP targets Cyclophilin B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclophilin B fusion protein Ag2161
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 19-24 kDa
GenBank accession numberBC032138
Gene symbol PPIB
Gene ID (NCBI) 5479
RRIDAB_2237519
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).

Protocols

Product Specific Protocols
WB protocol for Cyclophilin B antibody 11607-1-APDownload protocol
IHC protocol for Cyclophilin B antibody 11607-1-APDownload protocol
IF protocol for Cyclophilin B antibody 11607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells.

Authors - Pei-Hsuan Chen
humanWB,IHC

Am J Hum Genet

PPIB mutations cause severe osteogenesis imperfecta.

Authors - van Dijk Fleur S FS
mouseWB

Cell Prolif

OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy

Authors - Fan Zhao
humanWB,IF

Hum Mol Genet

Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes.

Authors - Pyott Shawna M SM
mouseWB

Cells

HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy

Authors - Lang Shi
human,mouseWB,IF

J Virol

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.

Authors - Watanabe Akira A

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ganesh (Verified Customer) (02-07-2024)

Whole cell extracts of HAP1 (human) cells were resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. Membrane was incubated overnight at 4 deg C with cyclophilin antibody (diluted in 5% Milk in TBS-T). I used this antibody as a loading control in this experiment. The signal was very quick on a film using regular ECL substrate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000 dilution
  • Cell Tissue Type: HAP1
Cyclophilin B Antibody Western Blot validation (1:5000 dilution dilution) in HAP1 (Cat no:11607-1-AP)
FH

Parijat (Verified Customer) (08-21-2023)

Works well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000