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Cyclophilin B Polyclonal antibody
Cyclophilin B Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 11607-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, U-937 cells, Raji cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, rat liver tissue
|Positive IHC detected in
|human lymphoma tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11607-1-AP targets Cyclophilin B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, monkey
|Host / Isotype
|Rabbit / IgG
|Cyclophilin B fusion protein Ag2161
|peptidylprolyl isomerase B (cyclophilin B)
|Calculated molecular weight
|216 aa, 24 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).
Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells.
Am J Hum Genet
PPIB mutations cause severe osteogenesis imperfecta.
OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy
Hum Mol Genet
Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes.
HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy
CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ganesh (Verified Customer) (02-07-2024)
Whole cell extracts of HAP1 (human) cells were resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. Membrane was incubated overnight at 4 deg C with cyclophilin antibody (diluted in 5% Milk in TBS-T). I used this antibody as a loading control in this experiment. The signal was very quick on a film using regular ECL substrate.
Parijat (Verified Customer) (08-21-2023)