PPP1R13L Recombinant antibody

• 

  WB analysis using 86044-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86044-1-RR (PPP1R13L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PPP1R13L Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86044-1-RR

Clone No.250633E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B like protein

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, PC-3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86044-1-RR targets PPP1R13L in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PPP1R13L fusion protein Ag13273
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC064913
• Gene Symbol: PPP1R13L
• Gene ID (NCBI): 10848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WUF5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53-mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression.