PPPDE2 Polyclonal antibody

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  WB analysis using 32194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32194-1-AP (PPPDE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PPPDE2 Polyclonal Antibody for WB, ELISA

Cat No. 32194-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FAM152B, EC:3.4.-.-, EC:3.1.2.22, Desumoylating isopeptidase 1, DeSI-1

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, MDA-MB-453 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32194-1-AP targets PPPDE2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPPDE2 fusion protein Ag37655
• Full Name: PPPDE peptidase domain containing 2
• Calculated Molecular Weight: 168 aa, 18 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC112179
• Gene Symbol: PPPDE2
• Gene ID (NCBI): 27351
• RRID: AB_3670220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6ICB0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPPDE2 belongs to N1pC/P60 superfamily of papain-like enzymes, which comprise an extremely diverse group of proteins originating from archaea, bacteria, bacteriophages, viruses, and eukaryotes with predicted protease, amidase, transglutaminases, or acyltransferase activity but often uncharacterized function (PMID: 12620121). PPPDE2 exhibits palmitoyl protein thioesterase (S-depalmitoylation) activity towards synthetic substrates 4-methylumbelliferyl-6-S-palmitoyl-beta-D-glucopyranoside and S-depalmitoylation probe 5 (DPP-5) (PMID:35427157).