PRAME Polyclonal antibody

PRAME Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11438-1-AP

Synonyms

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

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Validation Data Gallery

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  WB analysis using 11438-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11438-1-AP (PRAME antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11438-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11438-1-AP (PRAME Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11438-1-AP

  IP Result of anti-PRAME (IP:11438-1-AP, 3ug; Detection:11438-1-AP 1:300) with A549 cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells
• Positive IP detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Product Information

11438-1-AP targets PRAME in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRAME fusion protein Ag1906
• Full Name: preferentially expressed antigen in melanoma
• Calculated molecular weight: 509 aa, 58 kDa
• Observed molecular weight: 50-58 kDa
• GenBank accession number: BC014074
• Gene symbol: PRAME
• Gene ID (NCBI): 23532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.