PRAS40 Polyclonal antibody

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  WB analysis of MCF-7 using 21097-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21097-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21097-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21097-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21097-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21097-1-AP (PRAS40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PRAS40 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21097-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AKT1S1, 40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, Proline rich AKT1 substrate 1, Proline-rich AKT1 substrate 1

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

21097-1-AP targets PRAS40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRAS40 fusion protein Ag15355
• Full Name: AKT1 substrate 1 (proline-rich)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC051844
• Gene Symbol: PRAS40
• Gene ID (NCBI): 84335
• RRID: AB_2878811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96B36
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice; Authors - Xiaoxue Jiang
mouse	WB	J Neuroinflammation; Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain; Authors - Yun Li
human	WB	Front Oncol; The Molecular Landscape and Biological Alterations Induced by PRAS40-Knockout in Head and Neck Squamous Cell Carcinoma.; Authors - Gang Chen ; KO Validated