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PRDX1 Monoclonal antibody
PRDX1 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66820-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, U2OS cells, HEK-293 cells, Caco-2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, LNCaP cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66820-1-Ig targets PRDX1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse , rat samples.
|Tested Reactivity||Human, mouse , rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||PRDX1 fusion protein Ag8821|
|Full Name||peroxiredoxin 1|
|Calculated molecular weight||199 aa, 22 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC007063|
|Gene ID (NCBI)||5052|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).
Peroxiredoxin 1 interacts with TBK1/IKKε and negatively regulates pseudorabies virus propagation by promoting innate immunity.
Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice
EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
PILAR (Verified Customer) (08-24-2023)
Excellent customer service and fast shipment. Waiting for the experiment results!