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  • KD/KO Validated

PRDX1 Monoclonal antibody

PRDX1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse , rat and More (1)







Cat no : 66820-1-Ig


MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, HEK-293 cells, Caco-2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, LNCaP cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66820-1-Ig targets PRDX1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRDX1 fusion protein Ag8821
Full Name peroxiredoxin 1
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC007063
Gene Symbol PRDX1
Gene ID (NCBI) 5052
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).


Product Specific Protocols
WB protocol for PRDX1 antibody 66820-1-IgDownload protocol
IHC protocol for PRDX1 antibody 66820-1-IgDownload protocol
IF protocol for PRDX1 antibody 66820-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanWB, CoIP

J Virol

Peroxiredoxin 1 interacts with TBK1/IKKε and negatively regulates pseudorabies virus propagation by promoting innate immunity.

Authors - Lin Lv

Antioxidants (Basel)

Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

Authors - Ling Zhu


EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion

Authors - Zi-Jie Zhang

Mol Neurobiol

PRDX1 Interfering Peptide Disrupts Amino Acids 70-90 of PRDX1 to Inhibit the TLR4/NF-κB Signaling Pathway and Attenuate Neuroinflammation and Ischemic Brain Injury

Authors - Xiang-Yu Ma


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


PILAR (Verified Customer) (08-24-2023)

Excellent customer service and fast shipment. Waiting for the experiment results!

  • Applications: Western Blot