PRDX1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PRDX1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B2A2

Cat no : 66820-1-PBS

Synonyms

MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2

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Validation Data Gallery

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  WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66820-1-Ig (same clone as 66820-1-PBS)

  WB result of PRDX1 antibody (66820-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66820-1-Ig (same clone as 66820-1-PBS)

  U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 66820-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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  WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PRDX1 antibody (66820-1-Ig, Clone: 2B2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66820-1-PBS targets PRDX1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

• Tested Reactivity: Human, mouse , rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRDX1 fusion protein Ag8821
• Full Name: peroxiredoxin 1
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC007063
• Gene Symbol: PRDX1
• Gene ID (NCBI): 5052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).