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  • KD/KO Validated

peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 10545-2-AP

Synonyms

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX II, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, human brain tissue, mouse brain tissue, SMMC-7721 cells, human placenta tissue, C2C12 cells, PC-12 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10545-2-AP targets peroxiredoxin 2 in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen peroxiredoxin 2 fusion protein Ag0835
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003022
Gene symbol PRDX2
Gene ID (NCBI) 7001
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Protocols

Product Specific Protocols
WB protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IHC protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IF protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IP protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

JTC-801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3-kinase/protein kinase B signalling pathway.

Authors - Bufei Zhao
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
humanWB

Proteomes

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Authors - Henrick Horita
human,mouseWB,IP,CoIP

Free Radic Biol Med

Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.

Authors - Daria M Svistunova
humanWB

Aging (Albany NY)

PRDX2 removal inhibits the cell cycle and autophagy in colorectal cancer cells.

Authors - Xiangru Zheng
  • KD Validated
humanWB

Free Radic Res

Deficiency of Grx1 leads to high sensitivity of HeLaS3 cells to oxidative stress via excessive accumulation of intracellular oxidants including ROS.

Authors - Tingyi Zhao