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peroxiredoxin 2 Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 10545-2-AP

Featured Product KD/KO validated

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

peroxiredoxin 2 Antibody WB HeLa cells 10545-2-AP

WB result of peroxiredoxin 2 antibody (10545-2-AP, 1:60,000) with si-Control and si-peroxiredoxin 2 transfected HeLa cells.

peroxiredoxin 2 Antibody IHC human ovary tumor tissue 10545-2-AP

Immunohistochemical of paraffin-embedded human ovary tumor using 10545-2-AP(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens)

peroxiredoxin 2 Antibody WB HeLa cells 10545-2-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10545-2-AP( peroxiredoxin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

peroxiredoxin 2 Antibody IP HEK-293 cells 10545-2-AP

IP Result of anti-peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:1000) with HEK-293 cells lysate 2800ug.

peroxiredoxin 2 Antibody IF HepG2 cells 10545-2-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10545-2-AP(peroxiredoxin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, human brain tissue, SMMC-7721 cells, HepG2 cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human ovary tumor tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
peroxiredoxin 2 fusion protein Ag0835
Full name:
peroxiredoxin 2
Calculated molecular weight:
22 kDa
Observed molecular weight:
22 kDa
GenBank accession number:
BC003022
Gene ID (NCBI):
7001
Gene symbol
PRDX2
Synonyms
NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX II, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

Background

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)


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