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- KD/KO Validated
peroxiredoxin 2 Polyclonal antibody
peroxiredoxin 2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 10545-2-AP
|Positive WB detected in||HepG2 cells, HeLa cells, HEK-293 cells, human brain tissue, mouse brain tissue, SMMC-7721 cells, human placenta tissue, C2C12 cells, PC-12 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10545-2-AP targets peroxiredoxin 2 in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||peroxiredoxin 2 fusion protein Ag0835|
|Full Name||peroxiredoxin 2|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC003022|
|Gene ID (NCBI)||7001|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)
|Product Specific Protocols|
|WB protocol for peroxiredoxin 2 antibody 10545-2-AP||Download protocol|
|IHC protocol for peroxiredoxin 2 antibody 10545-2-AP||Download protocol|
|IF protocol for peroxiredoxin 2 antibody 10545-2-AP||Download protocol|
|IP protocol for peroxiredoxin 2 antibody 10545-2-AP||Download protocol|
|Click here to view our Standard Protocols|
JTC-801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3-kinase/protein kinase B signalling pathway.
Br J Pharmacol
Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.
Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.
Free Radic Biol Med
Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.
Aging (Albany NY)
PRDX2 removal inhibits the cell cycle and autophagy in colorectal cancer cells.
Free Radic Res
Deficiency of Grx1 leads to high sensitivity of HeLaS3 cells to oxidative stress via excessive accumulation of intracellular oxidants including ROS.