Peroxiredoxin 2 Polyclonal antibody

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  WB analysis using 10545-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10545-2-AP

  WB result of peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells.

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  IP experiment of HepG2 using 10545-2-AP

  IP result of anti-peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug.

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  IHC staining of human liver using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens).

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  IF Staining of HepG2 using 10545-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 10545-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10545-2-AP

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

PRDX2, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB, NKEF-B

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Tested Applications

• Positive WB detected in: HepG2 cells, C2C12 cells, PC-12 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 41 publications below
• IHC: See 12 publications below
• IF: See 7 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

10545-2-AP targets Peroxiredoxin 2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peroxiredoxin 2 fusion protein Ag0835
• Full Name: peroxiredoxin 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003022
• Gene Symbol: peroxiredoxin 2
• Gene ID (NCBI): 7001
• RRID: AB_2168202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32119
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
rat,mouse	WB,IF	Clin Transl Med; PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction; Authors - Hao Zhang
human	WB	Angiogenesis; Endothelial USP11 drives VEGFR2 signaling and angiogenesis via PRDX2/c-MYC axis; Authors - Can Chen
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB,IHC	Oncogene; PMID: 26500057; Authors - Y-H Park
mouse,human	WB,IP,CoIP	Free Radic Biol Med; Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.; Authors - Daria M Svistunova