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peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10545-2-AP

Synonyms

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX II, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA



Tested Applications

Positive WB detected inHepG2 cells, C2C12 cells, PC-12 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10545-2-AP targets peroxiredoxin 2 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen peroxiredoxin 2 fusion protein Ag0835
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003022
Gene symbol PRDX2
Gene ID (NCBI) 7001
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Protocols

Product Specific Protocols
WB protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IHC protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IF protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IP protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Oncogene

PMID: 26500057

Authors - Y-H Park
mouseIF

Stem Cells

Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells.

Authors - Sun-Uk Kim
humanWB

Antioxid Redox Signal

Direct Proteomic Mapping of Cysteine Persulfidation.

Authors - Ling Fu
ratWB

Redox Biol

The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.

Authors - Xubo Chen
humanWB

Free Radic Biol Med

Compartmentally scavenging hepatic oxidants through AMPK/SIRT3-PGC1α axis improves mitochondrial biogenesis and glucose catabolism.

Authors - Meiling Wu
mouseWB

Antioxid Redox Signal

Selenium status in diet affects acetaminophen-induced hepatotoxicity via interruption of redox environment.

Authors - Jing Li