PRDX3 Polyclonal antibody

PRDX3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55087-1-AP

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, Peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22



Tested Applications

Positive WB detected inMCF7 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

55087-1-AP targets PRDX3 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name peroxiredoxin 3
Calculated molecular weight 28 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberNM_006793
Gene symbol PRDX3
Gene ID (NCBI) 10935
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 55087-1-APDownload protocol
IHC protocol for PRDX3 antibody 55087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
ratWB

Cardiovasc Toxicol

Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.

Authors - Ying Li
humanWB

J Cell Biochem

Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.

Authors - Sulpizio Marilisa M
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima