PRDX3 Polyclonal antibody

PRDX3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 55087-1-AP

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, Peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

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Validation Data Gallery

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  WB analysis using 55087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55087-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55087-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55087-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55087-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55087-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55087-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55087-1-AP (Peroxiredoxin 3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55087-1-AP (Peroxiredoxin 3 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HepG2 cells, MCF-7 cells, HeLa cells, mouse liver tissue, mouse brain tissue, THP-1 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

55087-1-AP targets PRDX3 in WB,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: peroxiredoxin 3
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: NM_006793
• Gene Symbol: PRDX3
• Gene ID (NCBI): 10935
• RRID: AB_10860268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
rat	WB	Cardiovasc Toxicol; Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.; Authors - Ying Li
mouse	WB,IF	Respir Res; Peroxiredoxin 3 has a crucial role in the macrophage polarization by regulating mitochondrial homeostasis; Authors - Wenhui Huang