Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, HepG2 cells, MCF-7 cells, HeLa cells, mouse liver tissue, mouse brain tissue, THP-1 cells, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55087-1-AP targets PRDX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||peroxiredoxin 3|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||26-28 kDa|
|GenBank accession number||NM_006793|
|Gene ID (NCBI)||10935|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.
Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.
Br J Pharmacol
Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.
J Cell Biochem
Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.
Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.