Validation Data Gallery
|Positive WB detected in
|A375 cells, HepG2 cells, HEK-293 cells
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue, human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10703-1-AP targets PRDX4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|human, mouse, rat, pig
|Host / Isotype
|Rabbit / IgG
|PRDX4 fusion protein Ag1177
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.
|Product Specific Protocols
|WB protocol for PRDX4 antibody 10703-1-AP
|IHC protocol for PRDX4 antibody 10703-1-AP
|IF protocol for PRDX4 antibody 10703-1-AP
|IP protocol for PRDX4 antibody 10703-1-AP
|Click here to view our Standard Protocols
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