PRDX4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRDX4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10703-1-AP

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

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Validation Data Gallery

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  WB analysis using 10703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10703-1-AP (PRDX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10703-1-AP

  WB result of PRDX4 antibody (10703-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX4 transfected HepG2 cells.

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  IP experiment of HepG2 using 10703-1-AP

  IP Result of anti-PRDX4 (IP:10703-1-AP, 3ug; Detection:10703-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human colon cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10703-1-AP (PRDX4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10703-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (10703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10703-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PRDX4 antibody (10703-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, HEK-293 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Product Information

10703-1-AP targets PRDX4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRDX4 fusion protein Ag1177
• Full Name: peroxiredoxin 4
• Calculated molecular weight: 31 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC007107
• Gene symbol: PRDX4
• Gene ID (NCBI): 10549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin
human	WB	Antioxid Redox Signal; Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.; Authors - Xiao-Jing Shi
human	WB	Antioxid Redox Signal; Direct Proteomic Mapping of Cysteine Persulfidation.; Authors - Ling Fu
mouse	WB	Bioorg Chem; Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents.; Authors - Chenhao Xu
	WB,IHC,IF,IP	FEBS J; A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems.; Authors - Daria Sicari
mouse	IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst