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CoraLite® Plus 647-conjugated PRDX4 Polyclonal antibody

PRDX4 Polyclonal Antibody for FC(Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC(Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10703

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372



Tested Applications

Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10703 targets PRDX4 in FC(Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX4 fusion protein Ag1177
Full Name peroxiredoxin 4
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007107
Gene Symbol PRDX4
Gene ID (NCBI) 10549
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 PRDX4 antibody CL647-10703Download protocol
Standard Protocols
Click here to view our Standard Protocols