|Positive WB detected in||A375 cells, Jurkat cells, HEK-293 cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human colon cancer tissue, human pancreas cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10703-1-AP targets PRDX4 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PRDX4 fusion protein Ag1177|
|Full Name||peroxiredoxin 4|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC007107|
|Gene ID (NCBI)||10549|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.
|Product Specific Protocols|
|WB protocol for PRDX4 antibody 10703-1-AP||Download protocol|
|IHC protocol for PRDX4 antibody 10703-1-AP||Download protocol|
|IF protocol for PRDX4 antibody 10703-1-AP||Download protocol|
|IP protocol for PRDX4 antibody 10703-1-AP||Download protocol|
|FC protocol for PRDX4 antibody 10703-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Br J Pharmacol
Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.
Hepatobiliary Pancreat Dis Int
Comparative proteomic analysis of differentially expressed proteins in human pancreatic cancer tissue.
Can J Physiol Pharmacol
Galectin-3 inhibition attenuates doxorubicin-induced cardiac dysfunction by upregulating the expression of peroxiredoxin-4.
Proteomics Clin Appl
Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.
J Cell Biochem
Nucleophosmin regulates intracellular oxidative stress homeostasis via antioxidant PRDX6.
Clin Exp Immunol
Peroxiredoxins are involved in the pathogenesis of multiple sclerosis and neuromyelitis optica spectrum disorder.