• Featured Product
  • KD/KO Validated

CoraLite®488-conjugated PRDX4 Monoclonal antibody

PRDX4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

5B6F1

Cat no : CL488-60286

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60286-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60286 targets PRDX4 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRDX4 fusion protein Ag13401
Full Name peroxiredoxin 4
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC007107
Gene symbol PRDX4
Gene ID (NCBI) 10549
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

Protocols

Product Specific Protocols
IF protocol for CL488 PRDX4 antibody CL488-60286Download protocol
Standard Protocols
Click here to view our Standard Protocols