Tested Applications
| Positive WB detected in | Daudi cells, HepG2 cells, U-937 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17724-1-AP targets PRX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12118 Product name: Recombinant human Prx5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC110983 Sequence: MGLAGVCALRRSAGYILVGGAGGQSAAAAARRCSEGEWASGGVRSFSRAAAAMAPIKVGDAIPAVEVFEGEPGNKVNLAELFKGKKGVLFGVPGAFTPGCSKTHLPGFVEQAEALKAKGVQVVACLSVNDAFVTGEWGRAHKAEGKVRLLADPTGAFGKETDLLLDDSLVSIFGNRRLKRFSMVVQDGIVKALNVEPDGTGLTCSLAPNIISQL Predict reactive species |
| Full Name | peroxiredoxin 5 |
| Calculated Molecular Weight | 214 aa, 22 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC110983 |
| Gene Symbol | PRX5 |
| Gene ID (NCBI) | 25824 |
| RRID | AB_2168628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30044 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRX5 antibody 17724-1-AP | Download protocol |
| WB protocol for PRX5 antibody 17724-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PRDX5 antibody (Cat# 17724-1-AP) has 28 citations spanning journals including Nature Metabolism, Nature Communications, Cell Chemical Biology, FASEB Journal, Journal of Biological Chemistry, Human Molecular Genetics, Oncogenesis, British Journal of Cancer, and Phytomedicine. Research fields cover oxidative stress signaling, mitochondrial homeostasis, ferroptosis, cardiac ischemia-reperfusion injury, renal fibrosis, NASH, cancer (prostate, glioblastoma, hepatocellular carcinoma), neurodegeneration, aging, and immune regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice | ||
Phytomedicine Chaihuang Yishen Granule ameliorates mitochondrial homeostasis by upregulating PRDX5/TFAM axis to inhibit renal fibrosis in CKD
| ||
Chemosphere MitoQ ameliorates PM2.5-induced pulmonary fibrosis through regulating the mitochondria DNA homeostasis | ||
Antioxidants (Basel) Peroxiredoxins Play an Important Role in the Regulation of Immunity and Aging in Drosophila |
